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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
A combinatorial approach to chemical modification of subtilisin Bacillus lentus
E Plettner1, K Khumtaveeporn, X Shang
1Department of Chemistry, University of Toronto, Ontario, Canada.
Bioorganic & Medicinal Chemistry Letters
|January 5, 1999
Abstract:
The reaction between methanethiosulfonate reagents and cysteine mutants of subtilisin is quantitative and can be used to prepare chemically modified mutant enzymes (CMMs) with novel properties. The virtually unrestricted structural variations possible for CMMs presents a preparative and screening challenge. To address this, a rapid combinatorial method for preparing and screening the activities of CMMs has been developed.

