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In situ generated O-glycan core 1 structure as substrate for Gal(beta 1-3)GalNAc beta-1,6-GlcNAc transferase
G Dudziak1, S Zeng, E G Berger
1Forschungszentrum Jülich GmbH, Institut für Biotechnologie, Jülich, Germany.
Bioorganic & Medicinal Chemistry Letters
|January 5, 1999
Abstract:
beta-Galactosidase from bovine testes was used in a one pot reaction together with a recombinant beta-1,6-GlcNAc transferase for the synthesis of GlcNAc(beta 1-6)GalNAc(alpha 1-OBn) (core 6-Bn). The galactosidase, which reversibly links galactose via a (beta 1-3) linkage to N-acetylgalactosamine, provides the substrate for the GlcNAc transferase in situ. The synthesis was carried out with a yield > 90%.