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Micropipette system for sampling and injecting small volumes

S Schreiber1, M Stüben, P Kröhan

  • 1Institut für Physiologie, Ruhr-Universität Bochum, Germany. Soren.Schreiber@ruhr-uni-bochum.de

Journal of Neuroscience Methods
|January 5, 1999
PubMed
Summary

This study introduces a novel micropipette system for precise liquid handling. It uses thermal expansion for accurate sampling and injection of nanoliter volumes without damaging biological samples.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Instrumentation

Background:

  • Accurate and precise liquid handling is crucial in various scientific fields.
  • Existing methods for micro-volume liquid transfer can be complex or damage sensitive materials.

Purpose of the Study:

  • To develop a novel micropipette system for precise sampling and injection of preset liquid volumes.
  • To ensure the system is minimally invasive to biological samples.

Main Methods:

  • Utilized thermal expansion of an oil phase within the micropipette shaft.
  • Controlled liquid volumes by adjusting electrical current to heat or cool the micropipette shaft.
  • Tested the system for sampling and injecting volumes around 20 nanoliters (nl).

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Main Results:

  • The developed system accurately samples and injects preset volumes, with demonstrated capability for ~20 nl.
  • Volume precision is largely independent of the viscosity of the surrounding medium.
  • Crucially, the pipette tip remains at ambient temperature, preventing damage to biological tissues.

Conclusions:

  • The thermal expansion micropipette offers a precise and non-damaging method for micro-volume liquid handling.
  • This technology has potential applications in sensitive biological research and microfluidic devices.
  • The system's independence from fluid viscosity broadens its applicability across diverse experimental conditions.