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Extender components and surfactants affect boar sperm function and membrane behavior during cryopreservation

M J Pettitt1, M M Buhr

  • 1Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.

Journal of Andrology
|January 6, 1999
PubMed
Summary

Cryopreservation of boar sperm is improved with egg yolk and glycerol, while surfactants like Pluronic F-127 and Orvus ES Paste show potential in enhancing sperm function and membrane structure.

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Area of Science:

  • Animal Science
  • Reproductive Biology
  • Biochemistry

Background:

  • Boar sperm cryopreservation is crucial for artificial insemination.
  • Extender composition significantly impacts sperm viability and function post-thaw.
  • Understanding membrane dynamics during cryopreservation is key to improving sperm quality.

Purpose of the Study:

  • To evaluate the effects of extender components (egg yolk, glycerol, surfactants) on boar sperm function and membrane structure.
  • To assess the cryoprotective efficacy of new surfactants (Orvus ES Paste and Pluronic F-127).
  • To correlate changes in head plasma membrane fluidity with post-thaw sperm function.

Main Methods:

  • Boar sperm were cryopreserved in various BF5 extenders with different combinations of egg yolk, glycerol, and surfactants.

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  • Post-thaw sperm function was assessed by measuring total motility and adenosine triphosphate (ATP) content.
  • Head plasma membrane (HPM) fluidity was analyzed using fluorescent probes (cPNA, tPNA, DPH) to monitor different membrane domains.

    • Main Results:

    • Egg yolk plus glycerol significantly improved post-thaw motility and viability compared to other extenders.
    • Surfactants did not improve overall sperm function but OEP and Pluronic F-127 enhanced acrosome morphology combined with motility.
    • Cryopreservation altered the fluidity dynamics of specific HPM domains (tPNA and DPH), with significant correlations found between membrane structure and cell function.

    Conclusions:

    • Egg yolk and glycerol are essential for optimal boar sperm cryopreservation.
    • Specific surfactants may offer benefits for certain aspects of sperm quality, particularly acrosome integrity.
    • Head plasma membrane fluidity dynamics are affected by cryopreservation and are linked to the functional success of boar sperm in different extenders.