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Related Experiment Videos

Twenty-four-hour heart preservation using continuous cold perfusion and copper (II) complexes

F W Sellke1, H W Richter, G Dunphy

  • 1Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, 02215, USA.

The Journal of Surgical Research
|January 8, 1999
PubMed
Summary

Copper(II) chloride (Cu(II)Cl2) effectively preserved rat hearts during 24-hour cold storage, improving functional recovery and reducing oxidative damage. This suggests Cu(II) compounds can enhance organ preservation for transplantation.

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Area of Science:

  • Cardiology
  • Biochemistry
  • Transplantation Science

Background:

  • Long-term heart preservation leads to tissue damage from reactive oxygen species.
  • Inhibiting oxygen-derived free radicals can maintain heart function post-preservation.
  • Copper(II) complexes may inhibit free radical effects by dismutating superoxide.

Purpose of the Study:

  • To evaluate the efficacy of copper(II) compounds in preserving isolated rat hearts during 24-hour hypothermic storage.
  • To assess the impact of Cu(II)Cl2 and Cu(II)2Asp4 on heart function and oxidative stress markers.

Main Methods:

  • Isolated rat hearts were preserved for 24 hours using the Langendorff technique with a hypothermic, hyperkalemic solution.
  • Perfusion solutions were supplemented with either Cu(II)Cl2 or Cu(II)2Asp4 to assess their protective effects.

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  • Functional recovery (systolic pressure, coronary flow, dP/dt) and lipid peroxidation markers were measured.

    • Main Results:

    • Cu(II)Cl2 treatment significantly improved post-preservation heart function compared to controls.
    • Both Cu(II)Cl2 and Cu(II)2Asp4 reduced myocardial malonaldehyde and creatine kinase release, indicating less oxidative damage.
    • Cu(II)Cl2 demonstrated superior preservation of cardiac function over 24 hours.

    Conclusions:

    • Low-pressure, cold perfusion with enhanced solutions, including Cu(II)Cl2, is a promising method for donor heart preservation.
    • The protective effects of Cu(II)Cl2 are likely due to superoxide dismutase activity and lipid peroxidation inhibition.
    • Further research may explore the role of specific copper species and additives in mitigating reperfusion injury.