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Related Experiment Videos

Inducible site-specific recombination in the brain

C Kellendonk1, F Tronche, E Casanova

  • 1German Cancer Research Center, Mol. Biol. of the Cell I, Im Neuenheimer Feld 280, Heidelberg, 69120, Germany.

Journal of Molecular Biology
|January 8, 1999
PubMed
Summary

Researchers developed a new method for inducible gene targeting in the mouse brain using a modified Cre recombinase system. This allows for precise temporal control of genetic modifications, crucial for studying gene function in the central nervous system.

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Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • The Cre/loxP system enables tissue-specific somatic mutations in mice.
  • Temporal control of somatic mutagenesis is crucial for precise gene function analysis in the central nervous system.

Purpose of the Study:

  • To develop a temporally controlled gene targeting system for the mouse brain.
  • To compare ligand-regulated recombinases for inducible recombination.

Main Methods:

  • Fused Cre recombinase with ligand-binding domains (LBD) of progesterone receptor or estrogen receptor.
  • Utilized a Cre-LBD fusion protein activated by RU486, not progesterone.
  • Targeted expression to the brain using calcium-calmodulin-dependent kinase IIalpha or Thy-1 gene regulatory sequences.

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Main Results:

  • RU486 application induced Cre-mediated recombination of a lacZ reporter transgene in the cortex and hippocampus.
  • Demonstrated spatially and temporally controlled gene targeting in the brain.

Conclusions:

  • Developed an inducible Cre/loxP system for precise temporal control of gene manipulation in the mouse brain.
  • This system facilitates detailed analysis of gene function in the central nervous system.