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Updated: Jul 30, 2026
Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation
Published on: May 2, 2010
HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos
T Ishimoto1, A Kamei, S Koyanagi
1Department of Organic Materials, Osaka National Research Institute, Ikeda, Japan.
Abstract:
We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activity in vitro for the chick telencephalic neurons and spinal neurons. Partial amino acid sequencing and immunoblotting revealed that the protein was chick heat shock protein 90 (HSP90). Commercially available bovine HSP90 and recombinant chick HSP90 expressed in Escherichia coli also showed the same activity. Since HSP10 (GroES) and HSP60 (GroEL) exhibited no activity for neurite outgrowth in the same culture, this activity was specific to HSP90 among the heat shock proteins.
