Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The oligosaccharyltransferase complex from yeast

R Knauer1, L Lehle

  • 1Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, 93040 Regensburg, Germany.

Biochimica Et Biophysica Acta
|January 8, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying a newly discovered HLA-C allele, HLA-C*02:138.

HLA·2018
Same author

Glucosylation of sterols and polyprenolphosphate in the Golgi apparatus of Phaseolus aureus.

Planta·2014
Same author

Cell-cycle arrest of plant suspension cultures by tunicamycin.

Planta·2013
Same author

Congenital disorder of glycosylation type Ij (CDG-Ij, DPAGT1-CDG): extending the clinical and molecular spectrum of a rare disease.

Molecular genetics and metabolism·2012
Same author

Effect of pretransplant human leukocyte antigen antibodies detected by solid-phase assay on heart transplant outcomes.

Transplantation proceedings·2011
Same author

The yeast SEC20 gene is required for N- and O-glycosylation in the Golgi. Evidence that impaired glycosylation does not correlate with the secretory defect.

The Journal of biological chemistry·2001
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Oligosaccharyltransferase (OST) is crucial for N-glycosylation, a vital protein modification. Yeast studies reveal OST is a nine-protein complex essential for this conserved process in eukaryotes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • N-glycosylation is a critical, conserved modification for eukaryotic secretory and membrane proteins.
  • The core step involves transferring a Glc3Man9GlcNAc2 oligosaccharide from dolichyl phosphate to proteins in the endoplasmic reticulum.
  • This process is catalyzed by the enzyme oligosaccharyltransferase (OST).

Purpose of the Study:

  • To investigate the composition and function of the oligosaccharyltransferase (OST) enzyme.
  • To understand the molecular basis of N-glycosylation in yeast.
  • To explore the evolutionary conservation of OST structure and function.

Main Methods:

  • Biochemical analysis of enzyme activity.
  • Molecular genetic studies in yeast.

Related Experiment Videos

  • Analysis of protein sequence homologies between yeast and higher eukaryotes.

    • Main Results:

    • Identified nine distinct proteins as components of the yeast OST complex.
    • Demonstrated that these nine proteins assemble into a heterooligomeric membrane-bound complex.
    • Showed that the complex is required for optimal OST activity in vivo.
    • Confirmed significant sequence homologies between yeast and higher eukaryotic OST subunits.

    Conclusions:

    • The yeast OST complex comprises nine proteins essential for N-glycosylation.
    • OST complexes exhibit conserved organization across different eukaryotic species.
    • These findings provide novel insights into the multi-component nature and evolutionary conservation of OST.