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Flow cytometric analysis for enterotoxin exposed on Clostridium perfringens spores
H Kusunoki1, D Hu, R H Piyankarage
1Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.
The Journal of Veterinary Medical Science
|January 8, 1999
Abstract:
Flow cytometric method (FCM) with fluorescent-labeled anti-CPE antibody was applied to develop a rapid, specific, and convenient method to detect enterotoxin (CPE) exposed on the surface of spores of Clostridium perfringens. The results obtained indicate that FCM can specifically detect CPE exposed on C. perfringens spores for a short time. Thus, FCM is found to be a rapid, specific, and convenient assay method for detection of CPE exposed on C. perfringens spores, suggesting that it will be hopefully useful to diagnose food poisoning caused by C. perfringens.