Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of a multigenic plasmid vector for HCV DNA immunization

S Papa1, M Rinaldi, A Mangia

  • 1Laboratory for Molecular Oncology and Gene Therapy, IRCCS H. Casa Sollievo Sofferenza, San Giovanni Rotondo, FG, Italy.

Research in Virology
|January 8, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nitrous oxide and hypnotherapy in paediatric dentistry.

European journal of paediatric dentistry·2023
Same author

Electroporation as a cutting edge technique shaping the future of food processing: Comment on: "Advances in pulsed electric stimuli as a physical method for treating liquid foods" by Farzan Zare, Negareh Ghasemi, Nidhi Bansal, Hamid Hosano.

Physics of life reviews·2023
Same author

Dim artificial light at night alters gene expression rhythms and growth in a key seagrass species (Posidonia oceanica).

Scientific reports·2023
Same author

The connection between diverticulosis and colonic superficial neoplastic lesions in patients who underwent screening colonoscopy.

International journal of colorectal disease·2023
Same author

Clowntherapy in paediatric dentistry: The psychological approach to the paediatric patient.

European journal of paediatric dentistry·2022
Same author

Hydrothermal carbonization of the wet fraction from mixed municipal solid waste: Hydrochar characteristics and energy balance.

Waste management (New York, N.Y.)·2022

This study developed a novel DNA vaccine vector (pRC112-HCV) to enhance immune responses against Hepatitis C virus (HCV). The vector co-expresses an immunomodulatory cytokine and conserved HCV epitopes for a stronger, targeted immune response.

Area of Science:

  • * Immunology and Vaccinology
  • * Molecular Biology and Virology

Background:

  • * Hepatitis C virus (HCV) proteins like C, NS3, E1, and E2 are key targets for DNA vaccine development.
  • * Current anti-HCV DNA vaccines often induce weak or short-lived immune responses.
  • * Strategies to enhance DNA vaccine immunogenicity are crucial for effective HCV therapy.

Purpose of the Study:

  • * To develop an improved multigenic plasmid vector for enhanced anti-HCV DNA vaccination.
  • * To enable simultaneous expression of an immunomodulatory molecule and specific HCV antigenic domains.
  • * To direct host immune responses towards conserved and immunogenic HCV epitopes.

Main Methods:

  • * Development of a novel plasmid vector (pRC112-HCV) based on a two-transcription cassette prototype (pRC100).

Related Experiment Videos

  • * Incorporation of murine interleukin-2 (mIL2) for immunomodulation.
  • * Inclusion of a highly conserved antigenic domain of the HCV NS3 C-terminus (nt 4403-4829) containing B and T cell epitopes.

    • Main Results:

    • * The pRC112-HCV vector allows independent and simultaneous expression of mIL2 and the targeted HCV NS3 domain.
    • * The vector is designed to combine local production of an immunomodulatory cytokine with targeted antigen presentation.
    • * This approach aims to elicit a stronger and more specific host immune response against HCV.

    Conclusions:

    • * The novel multigenic plasmid vector (pRC112-HCV) represents a promising strategy for enhancing anti-HCV DNA vaccine efficacy.
    • * Co-expression of mIL2 and specific HCV epitopes can modulate and strengthen the host immune response.
    • * This tailored approach holds potential for developing more effective vaccines against Hepatitis C virus.