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Related Experiment Videos

Chicken Eyes absent 2 gene: isolation and expression pattern during development

N Mishima1, S Tomarev

  • 1Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20892-2730, USA.

The International Journal of Developmental Biology
|January 8, 1999
PubMed
Summary
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Chicken Eyes absent 2 (Eya2) gene expression was studied in developing embryos. Eya2 plays a role in the development of various tissues, including the nervous system, eyes, and limbs.

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Comparative Genomics

Background:

  • Vertebrates possess at least three genes homologous to the Drosophila eyes absent (eya) gene.
  • The Eyes absent 2 (Eya2) gene is conserved across species, with chicken Eya2 showing 78-82% identity to mouse and human Eya2.

Purpose of the Study:

  • To isolate and characterize chicken Eyes absent 2 (Eya2) cDNA.
  • To investigate the expression pattern of chicken Eya2 during embryonic development.
  • To explore the potential role of Eya2 in the induction and patterning of various embryonic tissues.

Main Methods:

  • Isolation of chicken Eya2 cDNA from embryonic chicken lenses.
  • Elucidation of the open reading frame and protein size (59 kDa).
  • Analysis of Eya2 mRNA expression patterns using developmental stages (Hamburger and Hamilton stages) and specific tissues.

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Main Results:

  • Chicken Eya2 cDNA was successfully isolated, revealing a 59 kDa protein with moderate conservation to mammalian orthologs.
  • Eya2 exhibited dynamic expression throughout embryonic development, detected early in the foregut (stage 9).
  • Expression was observed in neural crest derivatives, cranial placodes (nasal pit at stage 13), and developing eye structures (neural retina at stage 24, lens around day 9), as well as cornea and iris.

Conclusions:

  • Chicken Eya2 is expressed early in the nasal placode, suggesting a role in its induction.
  • Eya2 expression in the eye, cornea, and iris supports its involvement in ocular development.
  • Expression in wing and limb buds indicates a role for Eya2 in limb connective tissue patterning.