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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 21, 2010
Occurrence of cello-oligosaccharides in the apoplast of auxin-treated pea stems
1Wood Research Institute, Kyoto University, Gokasho Uji, Kyoto 611, Japan (R.T., F.S., T.H.).
Abstract:
Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cello-oligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotetraose after subsequent thin-layer chromatography and paper electrophoresis. Cello-oligosaccharides in the apoplastic fraction were monitored using cellobiose dehydrogenase. Both xyloglucan and cello-oligosaccharides appeared to be formed concurrently within 30 min after treatment with the auxin, and the cello-oligosaccharides increased with stem elongation even after 2 h. The total activity of cellulase did not increase for up to 4 h.
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