Related Experiment Videos
Spreading depression-induced gene expression is regulated by plasma glucose
J Koistinaho1, S Pasonen, J Yrjänheikki
1A.I. Virtanen Institute, University of Kuopio, Finland. jari.koistinaho@uku.fi
Stroke
|January 8, 1999
Summary
Plasma glucose levels significantly impact gene expression changes during spreading depression (SD), a key factor in brain ischemia. Glucose availability modulates the induction of specific genes, influencing neuronal damage.
Area of Science:
- Neuroscience
- Molecular Biology
- Ischemia Research
Background:
- Plasma glucose and spreading depression (SD) are critical determinants of brain ischemia.
- Understanding their interplay is crucial for neuroprotection strategies.
Purpose of the Study:
- To investigate how plasma glucose levels influence gene expression induced by spreading depression (SD) in the brain cortex.
Main Methods:
- SD was induced using KCl application.
- Hyperglycemia and hypoglycemia were achieved via glucose and insulin injections.
- Gene expression of c-fos, COX-2, PKCdelta, and HO-1 was analyzed using in situ hybridization.
Main Results:
- SD alone strongly induced c-fos, COX-2, HO-1, and PKCdelta.
- Hypoglycemia alone did not induce significant gene expression, while hyperglycemia minimally induced c-fos.
- Both hypoglycemia and hyperglycemia altered SD-induced gene expression patterns, with differential effects on early- and late-response genes.
Conclusions:
- Plasma glucose availability is a key regulator of SD-induced gene expression.
- Glucose differentially affects early-response (c-fos, COX-2) and late-response genes (PKCdelta, HO-1).
- Altered glucose metabolism may contribute to neuronal damage by modulating gene expression during ischemic events.