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[Clinical evaluation of platelet function analyzer PFA-100 in dogs]
Berliner Und Munchener Tierarztliche Wochenschrift
|January 9, 1999
Summary
The platelet function analyzer PFA-100 shows promise for canine platelet function testing, particularly with the collagen/ADP cartridge, but its clinical usability is limited by factors like hematocrit variations and acetylsalicylic acid administration.
Area of Science:
- Veterinary Hematology
- Platelet Function Analysis
- Hemostasis Research
Context:
- The PFA-100 analyzer measures in vitro bleeding time using collagen/ADP or collagen/epinephrine cartridges.
- Assessing platelet function is crucial for diagnosing and managing bleeding disorders in dogs.
Purpose:
- To evaluate the applicability of the PFA-100 analyzer for canine blood samples.
- To establish reference ranges for healthy dogs and assess sensitivity in thrombocytopenic dogs.
Summary:
- Reference ranges for closure time and blood volume were established for healthy dogs using both collagen/ADP (47-81s, 223-303µL) and collagen/epinephrine (67-210s, 269-611µL) cartridges.
- The collagen/ADP cartridge demonstrated higher sensitivity for detecting platelet function abnormalities in thrombocytopenic dogs.
- Moderate correlation found between in vitro and in vivo bleeding times (rs=0.590).
- Acetylsalicylic acid prolonged closure time, and low hematocrit (<25%) significantly affected results.
Impact:
- The PFA-100 analyzer shows potential for canine platelet function assessment, especially with the collagen/ADP cartridge.
- Clinical usability is restricted due to sensitivity to hematocrit levels and drug effects.
- Further research may refine its application in veterinary diagnostics.