An acrylic ampoule represents a denture surface in a microcalorimetric biofilm problem

An acrylic microcalorimetric ampoule with inserts represented a denture surface in a Streptococcus mutants biofilm study. The biofilm of micro-organisms did not contain as many micro-organisms as those previously studied in planktonic suspension. A more sensitive microcalorimeter (Thermometric's thermal activity monitor) was required to detect the metabolism of S. mutants adhered to an engineered acrylic flow-through ampoule. Biofilm heat outputs were obtained in the acrylic ampoule following standardized procedures with cryopreserved aliquots of a homogeneous suspension of S. mutants.