Clozapine does activate nigrostriatal dopamine neurons in unanesthetized rats

Antipsychotic drugs are traditionally classified as typical or atypical on the basis of their property to cause or not to cause extrapyramidal side-effects. A widely accepted selectivity for the mesolimbic, vs. the nigrostriatal, dopaminergic system is postulated to underlie the existence of fewer or no extrapyramidal side-effects during treatment with atypical neuroleptics. In order to verify this hypothesis we examined the effect of acute clozapine on nigrostriatal dopaminergic neurons recorded from non-anaesthetised and from chloral hydrate-anaesthetised rats. Extracellular single-unit recording coupled with antidromic activation from the neostriatum was used. Intravenous administration of cumulative doses of clozapine (1.25-10 mg/kg) increased the firing rate of nigrostriatal dopaminergic neurons in non-anaesthetised rats, but failed to significantly modify the activity of the same units under chloral hydrate anesthesia. These results indicate that acute clozapine activates nigrostriatal dopamine cells in non-anaesthetised rats and cast doubts about a direct link between the lack of significant extrapyramidal side-effects and the selectivity of atypical neuroleptics, such as clozapine, for the mesolimbic dopamine system.