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Dominant lethal test with rats exposed to 1,3-dichloropropene by inhalation
B B Gollapudi1, F S Cieszlak, S J Day
1The Dow Chemical Company, Health and Environmental Research Laboratory, Midland, Michigan 48674, USA. bgollapudi@dow.com
Abstract:
The potential of 1,3-dichloropropene (1,3-DCP) to induce dominant lethal mutations in the germ cells of male CD rats following inhalation exposure was investigated. Groups of 11-week-old males (30 animals/group) were exposed to 1,3-DCP vapors by inhalation at targeted concentrations of 0 (negative control), 10, 60, and 150 ppm for 10 weeks (6 hr/day, 7 days/week). An additional group of 30 males (designated the pairfed group) was kept on dietary restriction for 10 weeks. This group served as a control for any effects of decreased feed consumption and the associated body weight loss on the dominant lethal indices in the males exposed to 1,3-DCP. At the termination of the exposures, each male was cohoused with naive adult virgin CD females for two consecutive mating trials (1 week/trial, 2 females/male). Females were necropsied 13 days after the conclusion of each weekly mating trial and the number of corpora lutea, live implantations, and resorptions were determined. There were no statistically significant increases in either the pre- or postimplantation embryonic/fetal loss in females mated with 1,3-DCP-exposed males relative to controls at any weekly mating period. Based on these results, it can be concluded that 1,3-DCP is not mutagenic to the male germ cells of CD rats at exposure levels < or = 150 ppm, the highest concentration tested.
Insights
This study investigated 1,3-dichloropropene (1,3-DCP) mutagenicity in male rats. Results show 1,3-DCP did not induce dominant lethal mutations in germ cells, even at high inhalation exposure levels.
Area of Science:
- Toxicology
- Genetics
- Reproductive Toxicology
Background:
- 1,3-dichloropropene (1,3-DCP) is an industrial chemical with potential health risks.
- Assessing its mutagenic potential in germ cells is crucial for understanding reproductive toxicity.
Purpose of the Study:
- To investigate the potential of 1,3-DCP to induce dominant lethal mutations in male CD rat germ cells.
- To evaluate the mutagenicity of 1,3-DCP following inhalation exposure at various concentrations.
Main Methods:
- Male CD rats were exposed via inhalation to 1,3-DCP (0, 10, 60, 150 ppm) for 10 weeks.
- Exposed males were mated with naive females, and reproductive endpoints (corpora lutea, implantations, resorptions) were analyzed.
- A pair-fed control group accounted for effects of reduced food intake and body weight loss.
Main Results:
- No statistically significant increases in pre- or postimplantation embryonic/fetal loss were observed.
- Females mated with 1,3-DCP exposed males showed no increased rates of fetal loss compared to controls.
- Mutagenic effects were not detected at any tested exposure level, up to 150 ppm.
Conclusions:
- 1,3-DCP is not mutagenic to the male germ cells of CD rats at exposure levels up to 150 ppm.
- The study provides evidence against the dominant lethal mutagenicity of 1,3-DCP in this model system.