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Related Experiment Videos

Time-resolved fluoroimmunoassay for plasma enterolactone

H Adlercreutz1, G J Wang, O Lapcík

  • 1Institute for Preventive Medicine, Nutrition and Cancer, Helsinki, Finland.

Analytical Biochemistry
|January 12, 1999
PubMed
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We developed a new assay to measure enterolactone, a biomarker for healthy diet intake, in blood. This method accurately quantifies enterolactone levels, aiding dietary assessments.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Nutritional Science

Background:

  • Enterolactone is a key biomarker indicating healthy diet adherence.
  • It is produced by gut bacteria from plant lignans found in fiber-rich foods.
  • Accurate quantification of enterolactone is crucial for dietary assessment.

Purpose of the Study:

  • To develop and validate a novel time-resolved fluoroimmunoassay (TR-FIA) for enterolactone determination in human plasma/serum.
  • To establish the assay's sensitivity, specificity, and precision.
  • To measure enterolactone levels in a Finnish population cohort.

Main Methods:

  • Synthesis of 5'-O-carboxymethoxyenterolactone and its conjugation to bovine serum albumin for antigen production.
  • Development of a europium chelate-labeled tracer using the same enterolactone derivative.

Related Experiment Videos

  • Immunoassay performed after enzymatic hydrolysis and ether extraction, utilizing a VICTOR 1420 multilabel counter.

    • Main Results:

    • The assay demonstrated high specificity with no cross-reactivity against other lignans or flavonoids.
    • Intra-assay and inter-assay coefficients of variation were low (4.6-6.0% and 5.5-9.9%, respectively).
    • The assay's working range was 1.5-540 nmol/L, with measured enterolactone levels in 224 Finnish subjects.

    Conclusions:

    • The developed TR-FIA method is a reliable and specific tool for quantifying enterolactone in serum/plasma.
    • The assay provides valuable data for assessing dietary habits and health status.
    • Enterolactone levels in the studied Finnish population provide a reference range for future research.