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Helicobacter pylori: a eubacterium lacking the stringent response

G L Scoarughi1, C Cimmino, P Donini

  • 1Dipartimento di Biologia Cellulare e dello Sviluppo, Università "La Sapienza," 00185 Rome, Italy.

Journal of Bacteriology
|January 12, 1999
PubMed
Summary
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Helicobacter pylori exhibits a relaxed phenotype, accumulating 16S rRNA without increased guanosine polyphosphates during amino acid starvation. This unique characteristic challenges typical stringent control responses in bacteria.

Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Molecular Biology

Background:

  • The stringent response is a crucial bacterial survival mechanism involving guanosine polyphosphates (pppGpp and ppGpp) that regulates gene expression during stress.
  • Amino acid starvation typically triggers the stringent response, leading to the accumulation of 16S rRNA and guanosine polyphosphates.

Purpose of the Study:

  • To investigate the stringent response, specifically 16S rRNA accumulation and guanosine polyphosphate production, in wild-type Helicobacter pylori strains under amino acid starvation.
  • To determine if H. pylori exhibits a relaxed phenotype, deviating from the typical stringent response.

Main Methods:

  • Studying 16S rRNA accumulation during amino acid starvation.
  • Measuring guanosine polyphosphate (pppGpp and ppGpp) levels in both growing and starved H. pylori cells.

Related Experiment Videos

  • Analyzing three wild-type strains of Helicobacter pylori.

    • Main Results:

    • All three wild-type H. pylori strains displayed a relaxed phenotype, accumulating 16S rRNA without a significant rise in guanosine polyphosphates upon amino acid starvation.
    • This represents the first documented instance of a wild-type eubacterium exhibiting a relaxed phenotype.
    • Basal levels of guanosine polyphosphates were detected in both growing and starved cells, indicating the presence of the necessary enzymatic machinery for their production.

    Conclusions:

    • Helicobacter pylori possesses a unique relaxed phenotype, diverging from the canonical stringent response observed in many bacteria.
    • The findings suggest that stringent control may be more critical for bacteria in diverse environments than for those in protected niches.
    • Further research is needed to understand the evolutionary implications and ecological significance of this relaxed phenotype in H. pylori.