Age peculiarities of changes in the contractile function of isolated rat hearts during prolonged perfusion

The experiments on the isolated hearts from adult and old rats have shown that during first hours of prolonged perfusion the contractile function decreased to a greater degree in adult rats compared to the old. Following a 3-h perfusion, there occurred a marked fall of the contractile function in old animals. The creatine phosphokinase activity in a perfusate grew sharply in the old rats and changed insignificantly in the adult. The Na,K-ATPase got activated in adult rats, which was prevented by the preliminary injection of actinomycin D, and declined in the old. It appeared that adult hearts had a better endurance of prolonged perfusion and kept sustaining a definite level of performance almost twice as long as the old. Under prolonged perfusion conditions, there has been synthesized a regulatory factor (invertor) in the heart of adult animals, which reduced the myocardial contractile function. The latter was evidenced from a fact that perfusate from adult hearts 'donors' promoted the decrease of contractility of the adult hearts 'recipients'. The preliminary injection of actinomycin D to adult rats prevented a developing decrease of the contractile function but did not induce any changes in the old. This permits to conclude that synthesis of the regulatory peptide factor promotes an economization and adaptation of the adult animal heart.