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Related Experiment Videos

Penicillin transport from cerebrospinal fluid

I N Walters, P F Teychenne, L E Claveria

    Neurology
    |November 1, 1976
    PubMed
    Summary

    Penicillin G is actively removed from cerebrospinal fluid (CSF) via a transport system. Intraventricular probenecid inhibits this removal, increasing penicillin G concentrations in the CSF.

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    Area of Science:

    • Pharmacokinetics
    • Neuroscience
    • Drug transport

    Background:

    • Penicillin G is a crucial antibiotic, but its penetration into the central nervous system is limited.
    • Understanding the mechanisms governing drug movement in the cerebrospinal fluid (CSF) is vital for effective treatment of CNS infections.

    Purpose of the Study:

    • To investigate the transport of penicillin G out of the cerebrospinal fluid (CSF) in vivo.
    • To determine if probenecid affects the elimination of penicillin G from the CSF.

    Main Methods:

    • Continuous ventriculocisternal perfusion was performed in conscious rabbits.
    • Penicillin G concentrations were measured in perfusate collected from the cisterna magna.
    • The effect of intraventricular probenecid administration on penicillin G levels was assessed.

    Main Results:

    • Penicillin G demonstrated significant passage from CSF, with concentrations in the cisterna magna reaching 76.5% of ventricular input.
    • Addition of probenecid to the perfusion fluid resulted in a 15% increase in penicillin G concentration within the cisternal CSF.
    • This increase was statistically significant (p < 0.005), indicating probenecid's inhibitory effect.

    Conclusions:

    • An active transport system is responsible for the removal of penicillin G from the cerebrospinal fluid.
    • Intraventricular administration of probenecid effectively inhibits this active efflux mechanism.
    • These findings in conscious animals corroborate previous studies using anesthetized models and intravenous probenecid.

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