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Related Experiment Videos

CD45RA(bright)/CD11a(bright) CD8+ T cells: effector T cells

C Höflich1, W D Döcke, A Busch

  • 1Institute of Medical Immunology, University Hospital Charité, Campus Mitte, Berlin, Germany.

International Immunology
|January 14, 1999
PubMed
Summary
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A novel CD8+ T cell subset, identified as CD45RA+ and CD11a(bright), exhibits memory-like properties. This subset, increasing with age and viral infections, likely represents recently activated effector T cells.

Area of Science:

  • Immunology
  • T cell biology
  • Cellular immunology

Background:

  • Peripheral T cell differentiation into naive and memory cells is crucial for adaptive immunity.
  • CD45RA and CD11a expression are standard markers for distinguishing naive (CD45RA+ or CD11a(dim)) from memory (CD45RA- or CD11a(bright)) T cells.
  • A distinct CD8+ T cell subset expressing both CD45RA and CD11a(bright) has been observed, increasing with age and viral infections, but its function remains unclear.

Purpose of the Study:

  • To investigate the functional role and characteristics of the CD8+ T cell subset expressing CD45RA and CD11a(bright).
  • To determine if this subset exhibits memory-like properties and its potential implications in immune responses.

Main Methods:

  • Flow cytometry analysis to identify and quantify T cell subsets based on CD45RA and CD11a expression.

Related Experiment Videos

  • Intracellular cytokine staining for Interferon-gamma (IFN-gamma) and Tumor Necrosis Factor-alpha (TNF-alpha).
  • Assessment of perforin, CD95, CD28, and CD57 expression.
  • In vitro stimulation of naive CD8+ T cells to observe transient subset expression and changes in size and granularity.

    • Main Results:

    • The CD45RA+CD11a(bright) CD8+ T cell subset produces IFN-gamma and TNF-alpha, indicative of effector function.
    • This subset exhibits high perforin content and CD95 expression, similar to established memory T cells.
    • It is characterized by a high percentage of CD28- and CD57+ cells, increased size and granularity, and transiently appears after in vitro stimulation of naive CD8+ T cells.

    Conclusions:

    • The CD45RA+CD11a(bright) CD8+ T cell subset displays characteristics of memory-like T cells.
    • This subset likely represents recently activated effector T cells capable of interacting with CD80/CD86-negative tissue cells.
    • Its increase with age and viral infections suggests a role in chronic immune stimulation and potential age-related immune dysfunction.