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Monoclonal antibodies to quinine
A Kitjaroentham1, S Khusmith, J Laothavorn
1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
The Southeast Asian Journal of Tropical Medicine and Public Health
|January 14, 1999
Summary
Researchers developed new monoclonal antibodies (MAbs) for detecting quinine. These antibodies showed promising reactivity with quinine in patient blood samples, suggesting potential for diagnostic applications.
Area of Science:
- Immunology
- Analytical Chemistry
- Pharmacology
Background:
- Monoclonal antibodies (MAbs) are crucial tools in diagnostics and research.
- Developing specific MAbs for small molecules like quinine presents unique challenges.
- Accurate detection of quinine is important for therapeutic drug monitoring and toxicology.
Purpose of the Study:
- To produce and characterize monoclonal antibodies specific to quinine.
- To evaluate the specificity and cross-reactivity of the generated MAbs.
- To assess the potential utility of these MAbs for detecting quinine in biological samples.
Main Methods:
- Quinine was conjugated to bovine serum albumin (BSA) via a hemisuccinate intermediate using N,N'-disuccinimidyl carbonate (DSC).
- Hybridoma technology was employed for monoclonal antibody production using the quinine-BSA conjugate as an immunogen.
- Indirect ELISA was used for screening and specificity testing, including cross-reactivity analysis with BSA and mefloquine.
Main Results:
- Six IgG1 monoclonal antibodies against quinine-BSA were successfully produced.
- The MAbs exhibited slight cross-reactivity with mefloquine-BSA due to structural similarities.
- A selected MAb demonstrated enhanced reactivity with blood samples from quinine-treated patients compared to normal blood.
Conclusions:
- The developed monoclonal antibodies show specificity for quinine.
- These MAbs hold potential as diagnostic probes for detecting quinine in biological fluids.
- Further validation is warranted to confirm their utility in clinical settings.