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Related Experiment Videos

Diverse TCRs recognize murine CD1

S M Behar1, T A Podrebarac, C J Roy

  • 1Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|January 14, 1999
PubMed
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The study found that T cells recognizing CD1d, crucial for immune regulation and tumor rejection, utilize a diverse range of T cell receptors (TCRs), not just the previously known invariant type. This diversity suggests a more flexible immune system response to CD1d. Keywords: CD1d, T cells, T cell receptors, immune regulation, tumor rejection.

Area of Science:

  • Immunology
  • Molecular Biology
  • T cell biology

Background:

  • CD1d-restricted T cells are vital for immunoregulation and tumor rejection.
  • Previously, CD1d-reactive T cells were thought to possess a limited T cell receptor (TCR) repertoire, often invariant.
  • Murine CD1d1-reactive T cells typically express an invariant Valpha14-Jalpha281 TCR rearrangement.

Purpose of the Study:

  • To investigate the diversity of TCRs in CD1d1-reactive T cell clones.
  • To determine if CD1d1-reactive T cells express a broader TCR repertoire than previously understood.
  • To characterize the cytokine production of CD1d1-reactive T cells with diverse TCRs.

Main Methods:

  • Immunization of mice with CD1d1-bearing cells.
  • Isolation of CD1d1-reactive T cell clones via limiting dilution without NK1 preselection.

Related Experiment Videos

  • Analysis of TCR repertoire diversity, including V-J junction sequencing.
  • Assessment of cytokine production (IL-4, IFN-gamma, IL-10).

    • Main Results:

    • A diverse repertoire of TCRs was identified in CD1d1-reactive T cell clones.
    • Only a minority (3 of 10) of clones expressed the previously known invariant Valpha14-Jalpha281 TCR.
    • Diverse TCRs, including those with Valpha10, -11, -15, and -17, were found to mediate both Type 1 and Type 2 cytokine production.
    • CD1d1-reactive T cells with diverse TCRs produced IL-4, IFN-gamma, and IL-10.

    Conclusions:

    • Significant TCR diversity exists among T cells directly reactive to the CD1d1 protein.
    • CD1d interacts with a broad array of TCRs, challenging previous notions of a restricted repertoire.
    • This TCR diversity indicates substantial redundancy and flexibility in the immune system's CD1d-mediated functions.