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Related Experiment Videos

Acto-myosin drug effects and aqueous outflow function

D L Epstein1, L L Rowlette, B C Roberts

  • 1Department of Ophthalmology, Duke University Medical Center, Duke University Eye Center, Durham, North Carolina 27710, USA.

Investigative Ophthalmology & Visual Science
|January 15, 1999
PubMed
Summary

Myosin inhibition significantly increases aqueous humor outflow facility in the eye. This suggests myosin plays a key role in regulating the eye's drainage system, offering potential for new glaucoma therapies.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Cytoskeletal proteins like actin and tubulin are implicated as therapeutic targets in the trabecular meshwork for glaucoma.
  • Acto-myosin interactions are hypothesized to be crucial for maintaining ocular outflow function.

Purpose of the Study:

  • To investigate the effects of compounds interfering with acto-myosin function on the eye's outflow pathway.
  • To evaluate 2,3-butanedione 2-monoxime (BDM), H-7 (myosin light-chain kinase inhibitor), and latrunculin B (actin disruptor) in an ex vivo and in vitro model system.

Main Methods:

  • Porcine eyes were perfused under constant pressure, and human trabecular meshwork (HTM) and Schlemm's canal (SC) cells were cultured.
  • Cells and perfused eyes were treated with BDM, H-7, or latrunculin B, followed by staining for actin and tubulin and epifluorescence microscopy.

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Main Results:

  • BDM, H-7, and latrunculin B significantly increased outflow facility by 36%, 63%, and 72%, respectively.
  • H-7 and latrunculin B caused rapid loss of filamentous actin in cultured cells, while BDM also induced cell-cell detachment and shape changes.
  • No observed alterations in beta-tubulin staining across treatments.

Conclusions:

  • Interference with myosin function can lead to secondary disruptions in actin organization.
  • Myosin, potentially via phosphorylation, may play a regulatory role in ocular outflow, suggesting it as a therapeutic target for glaucoma.