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Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria
A Klausegger1, M Hell, A Berger
1Children's Hospital, General Hospital Salzburg, Salzburg, Austria.
Journal of Clinical Microbiology
|January 16, 1999
Abstract:
Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.