Related Experiment Videos
Protective effect of histidine on MPP+-induced hydroxyl radical generation in rat striatum
1Department of Pharmacology, Oita Medical University, 1-1, Hasama-machi, Oita 879-5593, Japan.tobata@oita-med.ac.jp
Abstract:
We investigated the efficacy of histidine on MPP+-induced hydroxyl radical (.OH) formation in extracellular fluid of rat striatum. Rats were anesthetized and sodium salicylate in Ringer's solution (0.5 nmol microl-1 min-1) was infused through a microdialysis probe to detect the generation of.OH as reflected by the nonenzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. MPP+ (5 mM) clearly produced an increase in.OH formation. However, histidine (25 mM) reduced the.OH formation by the action of MPP+. These results indicate that histidine protects MPP+-induced.OH formation in rat striatum.
Insights
Histidine effectively reduced hydroxyl radical (.OH) formation in rat brains caused by MPP+. This suggests histidine acts as a neuroprotective agent against oxidative stress in the striatum.
Area of Science:
- Neuroscience
- Biochemistry
- Pharmacology
Background:
- Oxidative stress, specifically hydroxyl radical (.OH) formation, is implicated in neurodegenerative processes.
- The neurotoxin MPP+ is known to induce oxidative damage in the brain.
Purpose of the Study:
- To investigate the neuroprotective effect of histidine against MPP+-induced hydroxyl radical (.OH) formation.
- To determine if histidine can mitigate oxidative stress in the rat striatum.
Main Methods:
- Utilized in vivo microdialysis in anesthetized rats to measure extracellular hydroxyl radical (.OH) formation.
- Assessed .OH generation by quantifying 2,3-dihydroxybenzoic acid (DHBA) formation, a nonenzymatic product.
- Administered MPP+ and histidine to the striatum and monitored .OH levels.
Main Results:
- MPP+ administration significantly increased hydroxyl radical (.OH) formation in the rat striatum.
- Co-administration of histidine (25 mM) markedly reduced the MPP+-induced increase in .OH formation.
- Histidine demonstrated a protective effect against MPP+-induced oxidative stress.
Conclusions:
- Histidine exhibits neuroprotective properties by inhibiting hydroxyl radical (.OH) generation.
- The findings suggest histidine may be a potential therapeutic agent for conditions involving MPP+-related oxidative neurotoxicity.