Related Experiment Videos
Neutrophil apoptosis and dysfunction in uremia
M Cendoroglo1, B L Jaber, V S Balakrishnan
1Department of Medicine, New England Medical Center Hospitals, Tufts University School of Medicine, Boston, Massachusetts, USA.
Journal of the American Society of Nephrology : JASN
|January 16, 1999
Summary
Neutrophils from patients with end-stage renal disease undergo accelerated apoptosis, leading to impaired function. Uremic plasma also increases apoptosis in healthy neutrophils, contributing to immune dysfunction in kidney disease.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Patients with end-stage renal disease (ESRD) exhibit high rates of bacterial infections, suggesting impaired immune cell function.
- Neutrophil dysfunction in ESRD is attributed to factors like uremic toxins, malnutrition, and dialysis treatments.
Purpose of the Study:
- To investigate the role of apoptosis in neutrophil dysfunction among patients with uremia.
- To determine if uremic plasma directly contributes to neutrophil apoptosis and dysfunction.
Main Methods:
- Neutrophils from ESRD patients and healthy controls were incubated with autologous plasma or fetal calf serum.
- Apoptosis was quantified using flow cytometry and transmission electron microscopy.
- Neutrophil function was assessed by measuring superoxide production and phagocytosis of Staphylococcus aureus.
Main Results:
- Uremic neutrophils showed significantly higher rates of apoptosis compared to normal neutrophils.
- Exposure to uremic plasma accelerated apoptosis in healthy neutrophils.
- Increased apoptosis correlated inversely with neutrophil superoxide production and phagocytic capacity.
Conclusions:
- Neutrophils from uremic patients undergo accelerated apoptosis in vitro.
- Uremic plasma actively promotes neutrophil apoptosis, mimicking the dysfunction seen in ESRD.
- Accelerated apoptosis is a key mechanism underlying neutrophil dysfunction in uremia.