Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of selenium on rat thioredoxin reductase activity: increase by supranutritional selenium and decrease by

M M Berggren1, J F Mangin, J R Gasdaka

  • 1Arizona Cancer Center, University of Arizona, Tucson 85724-5024, USA.

Biochemical Pharmacology
|January 16, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of cortical folds in the human brain: An attempt to review biological hypotheses, early neuroimaging investigations and functional correlates.

Developmental cognitive neuroscience·2023
Same author

Biomarkers of Age-Related Frailty and Frailty Related to Diseases: An Exploratory, Cross-Sectional Analysis from the MAPT Study.

The journal of nutrition, health & aging·2022
Same author

Physical Activity and Changes in White Matter Hyperintensities over Three Years.

The journal of nutrition, health & aging·2018
Same author

Optogenetic Tractography for anatomo-functional characterization of cortico-subcortical neural circuits in non-human primates.

Scientific reports·2018
Same author

Association between Red Blood Cells Omega-3 Polyunsaturated Fatty Acids and White Matter Hyperintensities: The MAPT Study.

The journal of nutrition, health & aging·2018
Same author

MAPT STUDY: A MULTIDOMAIN APPROACH FOR PREVENTING ALZHEIMER'S DISEASE: DESIGN AND BASELINE DATA.

The journal of prevention of Alzheimer's disease·2015
Same journal

Unraveling the multifaceted roles of the aryl hydrocarbon receptor in the central nervous system: neurogenesis, neural function, and disease.

Biochemical pharmacology·2026
Same journal

4-DMB, a diclofenac derivative, alleviates DSS-induced ulcerative colitis in mice by binding TNFR1 to inhibit inflammation.

Biochemical pharmacology·2026
Same journal

Calceolarioside B alleviates airway barrier dysfunction and inflammation via targeting P2Y<sub>6</sub>R in OVA-triggered asthma.

Biochemical pharmacology·2026
Same journal

The dual-faced pyruvate kinase M2 in tumors: From cytoplasmic metabolic gatekeeper to nuclear transcriptional coactivator.

Biochemical pharmacology·2026
Same journal

A [<sup>68</sup>Ga]-labeled PDGFRβ-targeting peptide PET probe for assessing MASH-related fibrosis in diet-induced preclinical models and human liver specimens.

Biochemical pharmacology·2026
Same journal

Targeting TAK1 to overcome cisplatin resistance in lung adenocarcinoma by rewiring the NF-κB and p53 signaling.

Biochemical pharmacology·2026
See all related articles

Selenium levels impact thioredoxin reductase activity, an enzyme crucial for cell growth and cancer. Dietary selenium affects enzyme activity, not protein levels, suggesting altered selenocysteine incorporation.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Nutritional Science

Background:

  • Thioredoxin reductase (TrxR) is a selenocysteine-containing enzyme essential for reducing thioredoxin.
  • Thioredoxin (Trx) stimulates cell growth and is overexpressed in human cancers.
  • TrxR's redox activity suggests a role in cell growth regulation.

Purpose of the Study:

  • To investigate the effect of dietary selenium on thioredoxin reductase activity and protein levels in rats.
  • To determine if selenium affects enzyme specific activity or protein synthesis.

Main Methods:

  • Rats were fed diets with varying selenium concentrations (<0.01, 0.1, 1.0 ppm) for up to 98 days.
  • Thioredoxin reductase activity was measured in various tissues.
  • Protein levels were assessed using western blotting, and selenium incorporation was confirmed with 75Selenium.

Related Experiment Videos

Main Results:

  • Selenium deficiency decreased TrxR activity in lung, liver, and kidney.
  • High selenium diets increased TrxR activity in kidney and lung, but not protein levels.
  • Increased activity in high-selenium fed rats correlated with enhanced selenocysteine incorporation, not protein synthesis.

Conclusions:

  • Dietary selenium significantly influences thioredoxin reductase activity in a tissue-specific manner.
  • Changes in TrxR activity are primarily due to alterations in specific enzyme activity, likely via selenocysteine incorporation, rather than changes in protein expression.