Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondrial function is differentially affected upon oxidative stress

S M Cardoso1, C Pereira, R Oliveira

  • 1Center for Neuroscience of Coimbra and Faculty of Medicine of Coimbra, University of Coimbra, Portugal.

Free Radical Biology & Medicine
|January 16, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cerebral blood velocity responses during and after sprint interval exercise in healthy young adults.

Physiological reports·2026
Same author

Comorbidity and Malnutrition as Key Determinants of Mortality in Elderly Haemodialysis Patients: A One-Year Observational Study.

Journal of cachexia, sarcopenia and muscle·2025
Same author

Total mesometrial resection for the treatment of cervical cancer - an exploratory study of feasibility, safety and oncological outcomes in developing countries.

Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti·2025
Same author

Editorial: Load and wellness monitoring in sports: the relationship between different metrics.

Frontiers in sports and active living·2025
Same author

Madelung's disease: A challenging diagnosis.

Semergen·2024
Same author

IGFBP5 in osteosarcoma tumorigenesis: Gene expression profile among metastatic and non-metastatic patients.

Gene·2024

Oxidative stress damages mitochondria by affecting specific respiratory chain complexes. Antioxidants like vitamin E, idebenone, and glutathione (GSH) protect against this damage, with varied effectiveness depending on the complex involved.

Area of Science:

  • Mitochondrial biochemistry
  • Neuroscience
  • Oxidative stress research

Background:

  • Mitochondrial dysfunction is implicated in various neurological disorders.
  • Oxidative stress is a key factor contributing to mitochondrial damage.
  • Synaptosomes are valuable models for studying neuronal mitochondrial function.

Purpose of the Study:

  • To investigate the mechanisms of mitochondrial damage induced by oxidative stress in synaptosomes.
  • To evaluate the protective effects of specific antioxidants against oxidative damage to mitochondrial respiratory chain complexes.

Main Methods:

  • Synaptosomes were treated with ascorbate/iron to induce oxidative stress.
  • Mitochondrial membrane integrity was assessed via electron microscopy and lactate dehydrogenase (LDH) leakage.

Related Experiment Videos

  • Activities of mitochondrial respiratory chain complexes (Cx I-V) and ATP levels were measured.

    • Main Results:

    • Ascorbate/iron treatment led to loss of synaptosome membrane integrity and decreased ATP levels.
    • Complexes II, III, and V activities were significantly reduced, while Complexes I and IV remained unchanged.
    • Vitamin E and idebenone protected membrane integrity, while reduced glutathione (GSH) protected Complexes II and V activity.

    Conclusions:

    • Oxidative stress differentially affects mitochondrial respiratory chain complexes.
    • Specific antioxidants offer protection against mitochondrial damage, with varied mechanisms.
    • GSH protects against damage to Complexes II and V, likely via protein thiol oxidation, while vitamin E and idebenone protect Complex III, involving lipid peroxidation.