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Related Experiment Videos

Ascorbate 6-palmitate protects human erythrocytes from oxidative damage

D Ross1, S Mendiratta, Z C Qu

  • 1Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-6303, USA.

Free Radical Biology & Medicine
|January 16, 1999
PubMed
Summary

Ascorbate 6-palmitate, an amphipathic derivative, binds to erythrocyte membranes and protects against oxidative damage. This compound remains on the cell surface, safeguarding alpha-tocopherol and membrane integrity from external oxidants.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Oxidative Stress Research

Background:

  • Lipid-soluble antioxidants like alpha-tocopherol are crucial for protecting cell membranes from oxidative damage.
  • Ascorbate (vitamin C) is a water-soluble antioxidant, but its direct membrane protection is limited.
  • Amphipathic derivatives of ascorbate may offer enhanced membrane localization and antioxidant activity.

Purpose of the Study:

  • To investigate the retention of ascorbate 6-palmitate in intact erythrocyte cell membranes.
  • To determine if ascorbate 6-palmitate protects erythrocytes from peroxidative damage.
  • To elucidate the mechanism and location of ascorbate 6-palmitate's protective effects.

Main Methods:

  • Dose-dependent binding assays of ascorbate 6-palmitate to erythrocytes.

Related Experiment Videos

  • Analysis of derivative retention during ghost membrane preparation.
  • Assessment of ascorbate 6-palmitate's interaction with cell-impermeant agents.
  • Electron paramagnetic resonance (EPR) spectroscopy to detect free radicals.
  • Measurement of alpha-tocopherol oxidation and cis-parinaric acid oxidation.

    • Main Results:

    • Ascorbate 6-palmitate binding to erythrocytes was dose-dependent and the derivative was retained during membrane preparation.
    • The compound localized to the extracellular surface of erythrocytes, susceptible to external agents.
    • Bound ascorbate 6-palmitate reduced ferricyanide and generated ascorbyl free radicals.
    • It protected membrane alpha-tocopherol and intercalated cis-parinaric acid from oxidation by external oxidants.

    Conclusions:

    • Ascorbate 6-palmitate effectively binds to and is retained on the exterior surface of human erythrocyte membranes.
    • This amphipathic ascorbate derivative provides significant protection against externally initiated oxidative damage to the cell membrane.
    • Ascorbate 6-palmitate may act by directly scavenging oxidants or by regenerating alpha-tocopherol within the membrane.