Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Serum Calmodulin Activity in Male Lead-exposed Workers

Xu1, Jin, Jiang

  • 1Department of Occupational Health, Shanghai Medical University, 200032 Shanghai, People's Republic of China.

International Journal of Occupational and Environmental Health
|January 16, 1999
PubMed
Summary

Lead exposure significantly reduces serum calmodulin (CaM) activity in male workers. This study found a biological threshold for lead inhibition of CaM activity below 50 µg/dL blood lead.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of a novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome in Henan Province, China, using real-time reverse transcription PCR.

Journal of medical microbiology·2013
Same author

Anorectal dysfunction following low anterior resection for rectal carcinoma: a comparison between handsewn and stapled anastomosis.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2013
Same author

Construction and expression of antibody targeted plasminogen activator*

Enzyme and microbial technology·2000
Same author

Numerical studies on the dynamics of the globally coupled maps with sequential updating

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·2000
Same author

Coherent resonance in a one-way coupled system

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·2000
Same author

Titanium(IV) chloride and the amine-promoted baylis-hillman reaction

Organic letters·2000

Area of Science:

  • Biochemistry
  • Occupational Health
  • Toxicology

Background:

  • Lead exposure is a significant occupational hazard affecting numerous body systems.
  • Calmodulin (CaM) is a crucial calcium-binding protein involved in cellular signaling.
  • Understanding lead's impact on CaM activity is vital for assessing its toxicological effects.

Purpose of the Study:

  • To investigate the relationship between occupational lead exposure and serum calmodulin (CaM) activity in male workers.
  • To determine the potential inhibitory effect of blood lead levels (BPb) on CaM activity.
  • To identify factors influencing CaM activity in lead-exposed individuals.

Main Methods:

  • Serum CaM activity was measured in 75 lead-exposed and 21 non-exposed male workers.

Related Experiment Videos

  • Blood lead concentrations (BPb), calcium, magnesium, copper, zinc, and free erythrocytic protoporphyrin (FEP) were quantified.
  • Serum samples underwent heat treatment and centrifugation before CaM activity assay.

    • Main Results:

    • Lead-exposed workers exhibited significantly higher BPb compared to controls.
    • Serum CaM activity was significantly lower in the high-exposure group (BPb ≥ 50 µg/dL) than in controls.
    • A negative dose-response relationship was observed between BPb and CaM activity, with inhibition occurring below 50 µg/dL BPb.

    Conclusions:

    • Occupational lead exposure can inhibit serum CaM activity in humans.
    • Lead, calcium, and magnesium levels are significant predictors of serum CaM activity.
    • The findings highlight a potential mechanism for lead toxicity involving CaM dysfunction.