[Properties of MTPN11 mycobacteriophage]

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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

E L Zhilenkov1, I G Shemiakin, V N Stepanshina

  • 1State Research Center of Applied Microbiology, Obolensk, Russia.

Mikrobiologiia
|January 19, 1999
PubMed

Insights

This study characterizes phage MTPH11, a bacteriophage used for mycobacteria identification. We detail its structure, adsorption, replication, and stability, providing key data for its application.

Area of Science:

  • Bacteriophage biology
  • Mycobacterial research
  • Microbial genetics

Context:

  • Phage MTPH11 is utilized for mycobacteria identification.
  • Limited information exists on MTPH11's characteristics.
  • Mycobacterium smegmatis ATCC607 serves as the host strain.

Purpose:

  • To present key characteristics of phage MTPH11.
  • To elucidate the phage's morphology and infection dynamics.
  • To determine the phage's stability and DNA composition.

Summary:

  • Phage MTPH11 exhibits a B1 morphotype with an isometric head and long, noncontractile tail.
  • Adsorption rate constant on Mycobacterium smegmatis is 6.6 x 10(-9) ml/min, with a latent period of 65 min and progeny of 30-40 virions.
  • The phage has a buoyant density of 1.520 g/cm3, is chloroform-susceptible, stable between pH 5-9, and possesses 63 mol% G+C DNA.

Impact:

  • Provides essential data for the effective use of MTPH11 in mycobacterial identification.
  • Contributes to the understanding of bacteriophage-host interactions in mycobacteria.
  • Establishes a foundation for further research into MTPH11 and related phages.

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