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[Properties of MTPN11 mycobacteriophage]
E L Zhilenkov1, I G Shemiakin, V N Stepanshina
1State Research Center of Applied Microbiology, Obolensk, Russia.
Abstract:
Some characteristics of the poorly studied phage MTPH11, which is used for identification of mycobacteria, are presented. The phage has an isometric head and a long noncontractile tail (B1 morphotype). The attachment apparatus of this phage includes a basal plate composed of two joint disks and a single tail fiber. The constant of phage adsorption on Mycobacterium smegmatis ATCC607 cells is 6.6 x 10(-9) ml/min. The latent infection period in the MTPH11-host strain 607 system in 65 min; phage progeny ranges from 30 to 40 virions per one cell. The constant of phage inactivation with a homologous antiserum is 50 min-1. The buoyant density of intact MTPH11 virion in CsCl amounts to 1.520 g/cm3. The phage is susceptible to chloroform, retains lytic activity within a pH range of 5 to 9, and is resistant to inactivating agents. The G + C content of the phage DNA is 63 mol%.
Insights
This study characterizes phage MTPH11, a bacteriophage used for mycobacteria identification. We detail its structure, adsorption, replication, and stability, providing key data for its application.
Area of Science:
- Bacteriophage biology
- Mycobacterial research
- Microbial genetics
Context:
- Phage MTPH11 is utilized for mycobacteria identification.
- Limited information exists on MTPH11's characteristics.
- Mycobacterium smegmatis ATCC607 serves as the host strain.
Purpose:
- To present key characteristics of phage MTPH11.
- To elucidate the phage's morphology and infection dynamics.
- To determine the phage's stability and DNA composition.
Summary:
- Phage MTPH11 exhibits a B1 morphotype with an isometric head and long, noncontractile tail.
- Adsorption rate constant on Mycobacterium smegmatis is 6.6 x 10(-9) ml/min, with a latent period of 65 min and progeny of 30-40 virions.
- The phage has a buoyant density of 1.520 g/cm3, is chloroform-susceptible, stable between pH 5-9, and possesses 63 mol% G+C DNA.
Impact:
- Provides essential data for the effective use of MTPH11 in mycobacterial identification.
- Contributes to the understanding of bacteriophage-host interactions in mycobacteria.
- Establishes a foundation for further research into MTPH11 and related phages.