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Nonadheshive stationary organ culture of normal human urinary bladder mucosa
V Vatne1, L Fjellbirkeland, J Litlekalsoey
1Department of Surgery, Haukeland Hospital, University of Bergen, Norway.
Anticancer Research
|January 19, 1999
Abstract:
Cold cup biopsies from normal human bladder mucosa were taken at cystoscopy. They were cut into fragments of 300-450 microns and grown in suspension in microwells base coated with agar. Within the first 24 hours of culture the fragments were totally covered by epithelium. After 7 days of culture the fragments had a morphology mimicking normal urothelial mucosa with an epithelium resting on a basement membraned for six weeks in culture. The purpose of this study was to provide a model representative of normal human urothelial mucosa for further studies of various diseases affecting the urinary bladder.