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Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
Published on: November 14, 2016
Immunolocalization of transforming growth factor-beta s and type I and type II receptors in rat articular cartilage
K Fukumura1, S Matsunaga, T Yamamoto
1Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Japan.
Abstract:
The activities of TGF-beta s and their receptors (T beta R) in the articular cartilage have not been fully elucidated as yet. Temporal and spatial expression of TGF-beta s and their receptors were examined immunohistochemically in the articular cartilage of growing rats to clarify their role in chondrogenesis. TGF-beta 1, -beta 2 and -beta 3 were strongly expressed from 6 to 50 weeks in the superficial, transitional, and least mature zones. The expression of TGF-beta s in hypertrophic chondrocyte was weak except TGF-beta 1. T beta R-I and T beta R-II were co-expressed with the ligand in the superficial, transitional, and least mature zones throughout the growth phase. This study revealed that TGF-beta s in the articular cartilage plays a major role in the modulation of endochondral ossification ensuring the growth and maintenance of the chondrocyte lineage in articular cartilage.

