Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

LC/MS with a particle beam interface in forensic toxicology

E Spratt1, G M Vallaro

  • 1Westchester County Department of Laboratories and Research, Valhalla, New York, USA.

Clinics in Laboratory Medicine
|January 19, 1999
PubMed
Summary

This study highlights the use of high-pressure liquid chromatography/mass spectrometry (HPLC/MS) for screening forensic samples in postmortem and driving while intoxicated (DWI) cases. The method effectively detects various therapeutic and subtherapeutic drug levels, aiding toxicological analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid liquid chromatography/tandem mass spectrometer (LCMS) method for clozapine and its metabolite N-desmethyl clozapine (norclozapine) in human serum.

Journal of clinical laboratory analysis·2009
Same author

A fatality due to accidental PineSol ingestion.

Journal of analytical toxicology·2000
Same author

A fatality related to the veterinary anesthetic telazol.

Journal of analytical toxicology·1999
Same author

Children with both developmental and behavioral needs: profile of two clinic populations.

Child psychiatry and human development·1998
Same author

Bulimia and dextromethorphan abuse. A case study.

Journal of substance abuse treatment·1997
Same author

Normally saprobic cryptococci isolated from Cryptococcus neoformans infections.

Journal of clinical microbiology·1991

Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Forensic toxicology relies on various instruments for drug screening and confirmation.
  • High-pressure liquid chromatography/mass spectrometry (HPLC/MS) is a powerful technique for analyzing biological samples.

Purpose of the Study:

  • To evaluate the application of HPLC/MS for screening forensic postmortem and driving while intoxicated (DWI) cases.
  • To present limits of detection and extraction efficiencies for various compounds using this method.

Main Methods:

  • Utilized a high-pressure liquid chromatograph (HPLC) with a particle beam interface.
  • Coupled the HPLC to a mass spectrometer (MS) detector and a photodiode array detector.
  • Screened for a wide range of drugs including antidepressants, drugs of abuse, and common medications.

Related Experiment Videos

Main Results:

  • Demonstrated the capability of HPLC/MS to detect therapeutic and subtherapeutic levels of selected drugs.
  • Provided limits of detection and extraction efficiencies for numerous compounds.
  • Illustrated practical application through case discussions of various drug combinations.

Conclusions:

  • HPLC/MS is an effective screening tool for forensic toxicology in postmortem and DWI investigations.
  • The method allows for the identification of diverse drug classes and combinations.
  • This technique enhances the accuracy and scope of toxicological analyses.