A comparison of hair bundle mechanoreceptors in sea anemones and vertebrate systems

Hair bundle mechanoreceptors of the acousticolateralis system of vertebrates are similar to hair bundles found on tentacles of sea anemones, primitive marine invertebrates. In each case, hair bundles consist of actin-based stereocilia interconnected by extracellular linkages. Recently, considerable attention has been directed to one class of linkages called "tip links." Tip links interconnect the tip of one stereocilium to the adjacent, taller stereocilium. According to the currently favored hypothesis for signal transduction, tip links may be "gating springs" that gate cation channels opened during deflection of the hair bundle. To depolarize the membrane, deflections must be directed so as to induce strain on the tip links. Deflections in the opposite direction lead to hyperpolarization of the membrane. Hair cells adapt to prolonged deflection of hair bundles. Whereas in some vertebrates, adaptation is incomplete (i.e., the current fails to return to baseline), adaptation in anemones appears to be complete. Signal transduction is reversibly blocked by agents thought to interact with the transduction channel including streptomycin. In addition, signal transduction is abolished following exposure to agents thought to attack tip links including calcium-depleted buffers or elastase. Hair cells of lower vertebrates can be replaced by division and differentiation of supporting cells. In chickens, a repair system exists wherein tip links are replaced via a mechanism that does not involve protein synthesis. The repair mechanism of anemones involves synthesis of new proteins that may constitute replacement linkages and accessory proteins that attach the linkages to appropriate integral proteins.