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High-performance liquid chromatographic analysis of polyhydroxyflavones using solid-phase borate-complex extraction

H Tsuchiya1

  • 1Department of Dental Pharmacology, Asahi University School of Dentistry, Gifu, Japan. hiro@dent.asahi-u.ac.jp

Journal of Chromatography. B, Biomedical Sciences and Applications
|January 19, 1999
PubMed
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A new method precisely quantifies polyhydroxyflavones in food, wine, and blood. This solid-phase borate-complex extraction coupled with HPLC offers selective and efficient analysis for nutritional and pharmacokinetic studies.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • Polyhydroxyflavonoids are plant-derived compounds with potential health benefits.
  • Accurate quantification of these compounds in various matrices is crucial for research.

Purpose of the Study:

  • To develop a selective and efficient method for quantifying polyhydroxyflavonoids.
  • To validate the method for applications in nutritional and pharmacokinetic studies.

Main Methods:

  • Solid-phase borate-complex extraction using phenylboric acid cartridges.
  • High-performance liquid chromatography (HPLC) with diode array detection.
  • Simultaneous separation and quantification of six common polyhydroxyflavonoids.

Main Results:

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  • The method demonstrated high selectivity, separating target analytes without interference.
  • Absolute recoveries varied based on the diol structure of the flavonoids.
  • Quantification achieved in the range of 0.10-30.0 µg/ml with good accuracy and precision (CVs 0.8-10.2%).

Conclusions:

  • The developed method is suitable for the selective quantitation of polyhydroxyflavonoids.
  • It is applicable to complex biological and food samples like vegetables, red wine, and human blood plasma.
  • The method supports nutritional and pharmacokinetic investigations of polyhydroxyflavonoids.