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Peroxidase-antiperoxidase complex. Binding by Mallory bodies in unfixed tissue
Archives of Pathology & Laboratory Medicine
|October 1, 1976
Summary
Peroxidase-antiperoxidase (PAP) complexes selectively bind to Mallory bodies (MBs), aiding their identification in liver disease. This method is effective for detecting Mallory bodies in tissue samples and isolated fractions.
Area of Science:
- Hepatology
- Immunohistochemistry
- Biochemistry
Background:
- Mallory bodies (MBs) are protein aggregates found in hepatocytes.
- Accurate identification of MBs is crucial for diagnosing certain liver diseases.
Purpose of the Study:
- To investigate the selective binding of peroxidase-antiperoxidase (PAP) complexes to Mallory bodies.
- To establish a reliable method for identifying MBs in liver tissue and isolated fractions.
Main Methods:
- Incubation of liver tissue sections and isolated fractions with PAP complexes.
- Immunohistochemical staining to visualize PAP binding.
- Comparison with control tissue sections.
Main Results:
- Consistent and specific binding of PAP to MBs was observed in liver sections from patients with alcoholic cirrhosis and hepatoma.
- PAP also bound to isolated Mallory body fractions.
- No PAP staining was detected in control liver sections.
Conclusions:
- The PAP-binding method offers a reliable approach for the in situ and in vitro identification of Mallory bodies.
- The affinity of MBs for PAP may involve nonspecific protein-immunoglobulin interactions.