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Real-time control of purified product collection during chromatography of recombinant human insulin-like growth
R L Fahrner1, P M Lester, G S Blank
1Department of Recovery Sciences, Genentech, Inc., South San Francisco, CA 94080, USA. fahrner.robert@gene.com
Journal of Chromatography. A
|January 23, 1999
Abstract:
During preparative reversed-phase chromatography of recombinant human insulin-like growth factor-I (IGF), the separation of IGF from IGF aggregates cannot be determined using UV absorbance. An on-line reversed-phase chromatographic assay was developed that provides a quantitative measurement of IGF and IGF aggregates every 4 min, allowing real-time control of purified IGF collection. Process control using the on-line assay is a reliable and accurate method to collect purified IGF.