Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rat interleukin-4 assays

F Ramírez1, P Stumbles, M Puklavec

  • 1MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, UK. framirez@molbiol.ox.ac.uk

Journal of Immunological Methods
|January 23, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for the Higgs Boson Decay to a Z Boson and a Photon at the LHC.

Physical review letters·2024
Same author

Probing Small Bjorken-x Nuclear Gluonic Structure via Coherent J/ψ Photoproduction in Ultraperipheral Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2024
Same author

Measurement of the top quark mass using a profile likelihood approach with the lepton + jets final states in proton-proton collisions at <math></math>.

The European physical journal. C, Particles and fields·2023
Same author

Observation of τ Lepton Pair Production in Ultraperipheral Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2023
Same author

A search for decays of the Higgs boson to invisible particles in events with a top-antitop quark pair or a vector boson in proton-proton collisions at <math></math>.

The European physical journal. C, Particles and fields·2023
Same author

Measurement of the Dependence of the Hadron Production Fraction Ratios f_{s}/f_{u} and f_{d}/f_{u} on B Meson Kinematic Variables in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2023
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

This study presents methods to quantify rat interleukin-4 (IL-4) protein. The most effective method for measuring rat IL-4 protein is a bioassay using Major Histocompatibility Complex (MHC) class II expression on B cells.

Area of Science:

  • Immunology
  • Protein analysis

Background:

  • Interleukin-4 (IL-4) is crucial for immune responses.
  • Measuring IL-4 mRNA via RT-PCR does not reflect protein synthesis.
  • Accurate quantification of rat IL-4 protein is needed.

Purpose of the Study:

  • To develop and evaluate methods for measuring rat IL-4 protein.
  • To compare bioassays and ELISA for rat IL-4 detection.

Main Methods:

  • Developed bioassays using rat IL-4’s ability to induce T cell proliferation and MHC class II expression on B cells.
  • Utilized monoclonal antibody (mAb) OX-81 to inhibit rat IL-4 activity.
  • Developed a sandwich ELISA using a second anti-rat IL-4 mAb.

Main Results:

Related Experiment Videos

  • Bioassays demonstrated specificity, sensitivity, and reproducibility.
  • ELISA was satisfactory but less sensitive than bioassays.
  • The bioassay measuring MHC class II expression on B cells was identified as the preferred method.

    • Conclusions:

    • Bioassays are effective for quantifying rat IL-4 protein.
    • The B cell MHC class II expression bioassay is the recommended technique for rat IL-4 determination due to its superior performance.