Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Equilibrium binding of thrombin to platelets

B M Martin, W W Wasiewski, J W Fenton

    Biochemistry
    |November 2, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ross River virus transmission, infection, and disease: two and a half decades of research progress-an updated cross-disciplinary review.

    Clinical microbiology reviews·2026
    Same author

    Early barriers to neonatal porcine islet engraftment in a dual transplant model.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Age of first exposure to American football and long-term neuropsychiatric and cognitive outcomes.

    Translational psychiatry·2017
    Same author

    Dual islet transplantation modeling of the instant blood-mediated inflammatory reaction.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    Diagnostic specificity of a real-time RT-PCR in cattle for foot-and-mouth disease and swine for foot-and-mouth disease and classical swine fever based on non-invasive specimen collection.

    Veterinary microbiology·2008
    Same author

    Primary local orbital amyloidosis: biochemical identification of the immunoglobulin light chain kappaIII subtype in a small formalin fixed, paraffin wax embedded tissue sample.

    Journal of clinical pathology·2005
    Same journal

    Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

    Biochemistry·2026
    Same journal

    Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

    Biochemistry·2026
    Same journal

    Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

    Biochemistry·2026
    Same journal

    Discovery of Bacterial Unspecific Peroxygenases.

    Biochemistry·2026
    Same journal

    Lactate Biology: Subcellular Routing and Chemical Form Define Function.

    Biochemistry·2026
    Same journal

    Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

    Biochemistry·2026
    See all related articles

    Researchers studied how human [125I]thrombin binds to platelets to understand nonenzymic thrombin stimulation. They found complex binding equilibrium with high-affinity sites correlating to calcium secretion, crucial for platelet activation.

    Area of Science:

    • Hematology
    • Biochemistry
    • Cell Biology

    Background:

    • Thrombin plays a key role in platelet activation and hemostasis.
    • Understanding the nonenzymic interactions of thrombin with platelets is crucial for platelet stimulation research.

    Purpose of the Study:

    • To investigate the nonenzymic binding of human [125I]thrombin to human platelets.
    • To characterize the binding kinetics and affinity of thrombin to platelet receptors.

    Main Methods:

    • Studied binding of radio-iodinated (lactoperoxidase) human alpha-thrombin to washed human platelets.
    • Separated bound and free [125I]thrombin using oil centrifugation.
    • Analyzed binding data using double-reciprocal and Scatchard plots.

    Main Results:

    Related Experiment Videos

    • Maximum binding of [125I]thrombin to platelets occurred within 15 seconds.
    • Complex binding equilibrium was observed, dependent on thrombin concentration.
    • Identified 300-400 high-affinity binding sites (Kdiss = 1.8-2 nM) per platelet.
    • These sites correlate with thrombin-induced calcium secretion.

    Conclusions:

    • Human [125I]thrombin binds to platelets with high affinity.
    • The binding characteristics correlate with platelet stimulation, specifically calcium secretion.
    • This study elucidates key nonenzymic aspects of thrombin-platelet interactions.