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Updated: Aug 10, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
The recent origins of spliceosomal introns revisited
1Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia,B3H 4H7, Canada. jlogsdon@cs.dal.ca
Abstract:
Does the intron/exon structure of eukaryotic genes belie their ancient assembly by exon-shuffling or have introns been inserted into preformed genes during eukaryotic evolution? These are the central questions in the ongoing 'introns-early' versus 'introns-late' controversy. The phylogenetic distribution of spliceosomal introns continues to strongly favor the intronslate theory. The introns-early theory, however, has claimed support from intron phase and protein structure correlations.
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