Concentrations of energy substrates in oviduct fluid in unilaterally ovariectomised pigs

Nichol et al (1992, Journal of Reproduction and Fertility, 96, 699-707) identified a pre- to post-ovulatory decrease (approx 1 mM) in the amount of glucose in pig oviduct fluid. The present studies investigated whether the decrease was due to metabolism by embryos and/or oviduct tissues, and also whether there was a local influence of the ovary on the oviduct fluid content of energy substrates. Unilaterally ovariectomised pigs were used, in which, through compensation, oviducts that contained twice the normal number of embryos could be compared with oviducts which contained no embryos. Following unilateral ovariectomy and after two oestrous cycles of normal duration, surgery was performed 88 hours after the beginning of standing heat to obtain oviduct fluid samples, just before embryonic entry into the uterus. Luminal fluid samples from the ampulla and ampullary-isthmic junction from oviducts with and without an adjacent ovary were assayed for glucose, pyruvate and lactate concentrations. No significant differences were found between the glucose, pyruvate and lactate concentrations in fluids from the ampulla or ampullary-isthmic junction from oviducts containing embryos compared with absence of embryos (P > 0.05). Therefore, the post-ovulatory decrease was not due to the presence of embryos or to a local effect of the ipsilateral ovary. Consequently, pig oviduct fluid concentrations of glucose, lactate and pyruvate are seemingly regulated by systemic mechanisms.