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Related Experiment Videos

High density synthetic oligonucleotide arrays

R J Lipshutz1, S P Fodor, T R Gingeras

  • 1Affymetrix, Inc., Santa Clara, California 95051, USA. rob_lipshutz@affymetrix.com

Nature Genetics
|January 23, 1999
PubMed
Summary

Researchers developed GeneChip probe arrays using synthetic oligonucleotides for high-throughput genetic analysis. These arrays enable quantitative gene expression measurement, polymorphism discovery, and allele detection in cells and organisms.

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Area of Science:

  • Genomics and molecular biology
  • Bioengineering and nanotechnology

Background:

  • Experimental genomics utilizes sequence information to study gene, cell, and organism functions.
  • Advancements in high-throughput analysis are crucial for understanding complex biological systems.

Purpose of the Study:

  • To describe the fabrication and design of high-density, two-dimensional oligonucleotide arrays.
  • To showcase applications of these arrays in quantitative, parallel genetic and cellular analysis.

Main Methods:

  • Development of an approach using sequence information for designing synthetic oligonucleotide arrays.
  • Fabrication using spatially patterned, light-directed combinatorial chemical synthesis on a glass surface.
  • Creation of GeneChip probe arrays containing hundreds of thousands of unique oligonucleotides.

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Main Results:

  • GeneChip probe arrays enable quantitative and highly parallel measurements of gene expression.
  • The arrays facilitate the discovery of polymorphic loci.
  • Successful detection of thousands of alternative alleles is demonstrated.

Conclusions:

  • The developed GeneChip probe arrays offer a powerful tool for high-throughput genetic and cellular analysis.
  • This technology advances the capabilities of experimental genomics by enabling precise and large-scale molecular measurements.
  • The fabrication method allows for dense packing of diverse oligonucleotide probes for comprehensive biological studies.