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Related Experiment Videos

Preferential interactions of fluorescent probe Prodan with cholesterol

O P Bondar1, E S Rowe

  • 1Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66103, and Veterans Administration Medical Center, Kansas City, Missouri 64128 USA.

Biophysical Journal
|January 23, 1999
PubMed
Summary

The fluorescent probe Prodan

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Area of Science:

  • Membrane biophysics
  • Fluorescent probe applications
  • Lipid phase behavior

Background:

  • Prodan is a fluorescent probe used for studying model and biological membranes.
  • Prodan's fluorescence spectra indicate lipid phase states: liquid crystal (485 nm), gel (435 nm), and interdigitated gel (507 nm).

Purpose of the Study:

  • To investigate Prodan's fluorescent properties and partition coefficients in model membranes with varying lipid phase states and cholesterol content.
  • To determine if cholesterol affects Prodan's spectral response to lipid phase states.

Main Methods:

  • Studied fluorescent properties and partition coefficients of Prodan in phosphatidylcholine and phosphatidylethanol model membranes.
  • Varied lipid phase state and cholesterol content in model membranes.
  • Analyzed Prodan's emission maxima and partition coefficients under different conditions.

Main Results:

  • Cholesterol disrupts Prodan's ability to report on lipid phase state, causing spectra characteristic of the non-interdigitated gel phase (435 nm) regardless of the actual phase.
  • Cholesterol significantly increases Prodan's partition coefficients into lipid bilayers.
  • Prodan exhibits preferential interaction with cholesterol, indicating non-random distribution in cholesterol-containing membranes.

Conclusions:

  • Prodan is not a reliable reporter probe in membranes containing cholesterol due to preferential interactions.
  • Caution is advised when using Prodan in complex lipid mixtures, especially biological membranes, due to cholesterol interference.

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