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Updated: Aug 11, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
All accessible epitopes in the Salmonella lipopolysaccharide core are associated with branch residues
1Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. nnnalue@netscape.net
Abstract:
Antisera generated against each of the nine known chemotypes of Salmonella lipopolysaccharide (LPS) core were characterized in order to delineate cross-reactive epitopes and define the bases for their accessibility. Strongly cross-reactive epitopes were associated with three chemotypes: Ra and Rb4, which recognized alpha-GlcNAc-1-->2-alpha-Glc, and Rd1, which recognized L-alpha-D-heptose-1-->7-L-alpha-D-heptose. Both these disaccharides and the more weakly cross-reactive alpha-Gal-1-->6-alpha-Glc terminal in Rb3 LPS represent branch points along the core oligosaccharide. Therefore, branch points in endotoxin core oligosaccharides may generally be cross-reactive.
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