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Related Experiment Videos

Fgf10 is essential for limb and lung formation

K Sekine1, H Ohuchi, M Fujiwara

  • 1Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.

Nature Genetics
|January 23, 1999
PubMed
Summary

Fibroblast growth factor 10 (FGF10) is essential for lung and limb development. FGF10 deficiency in mice resulted in failed lung branching morphogenesis and complete limb truncation, confirming its critical role.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Fibroblast growth factors (FGFs) and their receptors mediate crucial cell interactions during embryonic development.
  • Fgf10's expression pattern suggests a regulatory role in brain, lung, and limb formation.

Purpose of the Study:

  • To elucidate the specific function of Fgf10 in embryonic development.
  • To investigate the consequences of Fgf10 deficiency on organogenesis and limb formation.

Main Methods:

  • Generation of Fgf10-deficient (Fgf10-/-) mice.
  • Phenotypic analysis of Fgf10-/- embryos and neonates.
  • Gene expression analysis in mutant limb buds.

Main Results:

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  • Fgf10-/- mice exhibited perinatal lethality due to absent lung development and disrupted pulmonary branching.
  • Mutant mice displayed complete truncation of forelimbs and hindlimbs, with initial limb bud formation but no outgrowth.
  • Key developmental structures like the apical ectodermal ridge (AER) and zone of polarizing activity (ZPA) failed to form in Fgf10-/- limb buds.

    • Conclusions:

    • Fgf10 is an indispensable regulator of lung development, specifically impacting branching morphogenesis.
    • Fgf10 is critical for limb bud outgrowth and the formation of essential signaling centers like the AER and ZPA.
    • This study establishes Fgf10 as a key genetic factor essential for both lung and limb formation during embryogenesis.