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An enzyme-based formaldehyde assay and its utility in a sponge sterol biosynthetic pathway
1Department of Chemistry and Biochemistry, Center for Molecular Biology and Biotechnology, Florida Atlantic University, Boca Raton, Florida 33431-0991, USA.
Journal of Natural Products
|January 23, 1999
Abstract:
An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge. The enzyme used in the assay, formaldehyde dehydrogenase, is NAD+ dependent, and the assay measures the production of NADH by determining the increase in fluorescence at 460 nm.