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Related Experiment Videos

Glucose sensor for low-cost lifetime-based sensing using a genetically engineered protein

L Tolosa1, I Gryczynski, L R Eichhorn

  • 1Department of Biophysics, Department of Chemical and Biochemical Engineering, University of Maryland, 725 West Lombard Street, Baltimore, Maryland, 21201, USA.

Analytical Biochemistry
|January 27, 1999
PubMed
Summary

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This study presents a novel glucose sensor using a modified glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The developed sensor accurately determines glucose concentration, offering a simple device approach for glucose sensing.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biophysics

Background:

  • Accurate glucose monitoring is crucial for diabetes management.
  • Existing glucose sensing technologies have limitations.
  • Development of novel, sensitive, and simple glucose sensors is needed.

Purpose of the Study:

  • To develop and characterize a novel glucose sensor.
  • To utilize a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry for glucose detection.
  • To assess the sensor's accuracy and potential for simple device implementation.

Main Methods:

  • Mutation of Escherichia coli GGBP to introduce a cysteine residue.
  • Labeling the mutant GGBP with 2-(4'-iodoacetamidoanilino)naphthalene-6-sulfonic acid (ANS).

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  • Development of a phase-modulation fluorometry sensor combining ANS-labeled GGBP with a ruthenium complex.

    • Main Results:

    • The ANS-labeled GGBP showed a twofold intensity decrease in response to glucose (Kd ~1 microM).
    • A phase-modulation sensor demonstrated a dramatic change in modulation upon glucose binding.
    • Modulation measurements at 2.1 MHz accurately determined glucose concentration.

    Conclusions:

    • A glucose sensor based on mutant GGBP and phase-modulation fluorometry is feasible.
    • The sensor exhibits sensitivity and accuracy for glucose determination.
    • This approach offers a pathway for developing simple and effective glucose sensing devices.